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GMNN Recombinant antibody

GMNN Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240835E6

Cat no : 84199-1-RR

Synonyms

Geminin, MGORS6, Gem, 240835E6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GMNN Recombinant antibody (84199-1-RR)

    IF Staining of HepG2 using 84199-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GMNN antibody (84199-1-RR, Clone: 240835E6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84199-1-RR

    Affinity and Kinetic Characterization of 84199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84199-1-RR against Human GMNN were performed. The affinity constant is 0.876 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84199-1-RR is GMNN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GMNN fusion protein Ag1076
Full Name geminin, DNA replication inhibitor
Calculated Molecular Weight 24 kDa
GenBank Accession NumberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

Protocols

Product Specific Protocols
IF protocol for GMNN antibody 84199-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols