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CD48 Recombinant antibody

CD48 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241389F9

Cat no : 84186-5-RR

Synonyms

CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1, BLAST

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD48 Recombinant antibody (84186-5-RR)

    WB analysis using 84186-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84186-5-RR (CD48 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84186-5-RR

    Affinity and Kinetic Characterization of 84186-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84186-5-RR against Human CD48 were performed. The affinity constant is 0.672 nM.

Tested Applications

Positive WB detected inRamos cells, Raji cells, Daudi cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84186-5-RR is CD48 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD48 molecule
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC016182
Gene Symbol CD48
Gene ID (NCBI) 962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.

Protocols

Product Specific Protocols
WB protocol for CD48 antibody 84186-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols