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4-1BBL/TNFSF9 Recombinant antibody

4-1BBL/TNFSF9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241386E7

Cat no : 84185-5-RR

Synonyms

TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 4-1BBL/TNFSF9 Recombinant antibody (84185-5-RR)

    WB analysis using 84185-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84185-5-RR (4-1BBL/TNFSF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using 4-1BBL/TNFSF9 Recombinant antibody (84185-5-RR)

    IF Staining of Raji using 84185-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using 4-1BBL/TNFSF9 antibody (84185-5-RR, Clone: 241386E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inDaudi cells, Raji cells, K-562 cells, HEK-293T cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84185-5-RR is 4-1BBL/TNFSF9 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor (ligand) superfamily, member 9
Calculated Molecular Weight 254 aa, 27 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC104805
Gene Symbol TNFSF9
Gene ID (NCBI) 8744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.

Protocols

Product Specific Protocols
WB protocol for 4-1BBL/TNFSF9 antibody 84185-5-RRDownload protocol
IF protocol for 4-1BBL/TNFSF9 antibody 84185-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols