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  • Western Blot (WB) analysis of various lysates using MED6 Recombinant antibody (84184-4-RR)

    WB analysis using 84184-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using MED6 Recombinant antibody (84184-4-RR)

    FC experiment of A431 using 84184-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84184-4-RR

    Affinity and Kinetic Characterization of 84184-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

MED6 Recombinant antibody

MED6 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84184-4-RR
Clone No.241382H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, Jurkat cells, mouse colon tissue, mouse brain tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84184-4-RR targets MED6 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED6 fusion protein Ag7541 Predict reactive species
Full Name mediator complex subunit 6
Calculated Molecular Weight 28 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
RRIDAB_3671738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Protocols

Product Specific Protocols
WB protocol for MED6 antibody 84184-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols