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Kininogen-1 Recombinant antibody, PBS Only (Capture)

Kininogen-1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241397G9

Cat no : 84175-4-PBS

Synonyms

KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01081-2

    Sandwich ELISA standard curve of MP01081-2

    Sandwich ELISA standard curve of MP01081-2, Human Kininogen-1 Recombinant Matched Antibody Pair - PBS only. 84175-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1162. 84175-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500pg/mL

  • Sample test of MP01081-2

    Sample test of MP01081-2

    Saliva of five individual healthy human donors was measured. The human Kininogen-1 concentration of detected samples was determined to be 1.55 ng/mL with a range of 0.70 - 2.43 ng/mL.

  • Sample test of MP01081-2

    Sample test of MP01081-2

    HepG2 (human hepatocellular carcinoma cells) was cultured in DMEM supplemented with 10% fetal bonive serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Kininogen-1, and measured 458.7 pg/mL.

  • Sample test of MP01081-2

    Sample test of MP01081-2

    Serum of six individual healthy human donors was measured. The human Kininogen-1 concentration of detected samples was determined to be 21,735.55 ng/mL with a range of 2,325.73 - 45,247.60 ng/mL.

  • Cytometric bead array standard curve of MP01081-1

    Cytometric bead array standard curve of MP01081-1

    Cytometric bead array standard curve of MP01081-1, Kininogen-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84175-4-PBS. Detection antibody: 84175-3-PBS. Standard: Eg1162. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01081-2

    Cytometric bead array standard curve of MP01081-2

    Cytometric bead array standard curve of MP01081-2, Kininogen-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84175-4-PBS. Detection antibody: 84175-2-PBS. Standard: Eg1162. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01081-3

    Cytometric bead array standard curve of MP01081-3

    Cytometric bead array standard curve of MP01081-3, Kininogen-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84175-4-PBS. Detection antibody: 84175-1-PBS. Standard: Eg1162. Range: 0.781-100 ng/mL.

Product Information

84175-4-PBS targets Kininogen-1 as part of a matched antibody pair:

MP01081-1: 84175-4-PBS capture and 84175-3-PBS detection (validated in Cytometric bead array)

MP01081-2: 84175-4-PBS capture and 84175-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01081-3: 84175-4-PBS capture and 84175-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name kininogen 1
Calculated Molecular Weight72kDa
GenBank Accession NumberNM_001102416.3
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.