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DPP3 Recombinant antibody

DPP3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241360D4

Cat no : 84173-3-RR

Synonyms

DPP III, Dipeptidyl peptidase III, Dipeptidyl peptidase 3, Dipeptidyl arylamidase III, Dipeptidyl aminopeptidase III

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP3 Recombinant antibody (84173-3-RR)

    WB analysis using 84173-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84173-3-RR (DPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84173-3-RR

    Affinity and Kinetic Characterization of 84173-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84173-3-RR against Human DPP3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84173-3-RR is DPP3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DPP3 fusion protein Ag1022
Full Name dipeptidyl-peptidase 3
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC007221
Gene Symbol DPP3
Gene ID (NCBI) 10072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPP3 (dipeptidyl-peptidase 3), also known as DPPIII, is one of the dipeptidyl aminopeptidases, that belongs to the peptidase M49 family. DPP3 is a metalloenzyme containing one Zn2+ ion in its active site. Furthermore, It was shown to have a novel zinc-binding motif ( HELLGH). DPP3 has been reported as an important enkephalin-degrading enzyme in the central nervous system, in human neutrophils. It has 3 isoforms produced by alternative splicing. Increased activity of DPP3 is associated with endometrial and ovarian cancers.

Protocols

Product Specific Protocols
WB protocol for DPP3 antibody 84173-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols