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PDCD4 Recombinant antibody

PDCD4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241463E3

Cat no : 84162-3-RR

Synonyms

Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDCD4 Recombinant antibody (84162-3-RR)

    WB analysis using 84162-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84162-3-RR (PDCD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using PDCD4 Recombinant antibody (84162-3-RR)

    FC experiment of HeLa using 84162-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDCD4 Recombinant antibody (84162-3-RR, Clone:241463E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84162-3-RR

    Affinity and Kinetic Characterization of 84162-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84162-3-RR against Human PDCD4 were performed. The affinity constant is 1.54 nM.

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, HUVEC cells, MCF-7 cells, HEK-293 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84162-3-RR is PDCD4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDCD4 fusion protein Ag3288
Full Name programmed cell death 4 (neoplastic transformation inhibitor)
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 54-64 kDa
GenBank Accession NumberBC026104
Gene Symbol PDCD4
Gene ID (NCBI) 27250
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.

Protocols

Product Specific Protocols
WB protocol for PDCD4 antibody 84162-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols