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ATG4C Recombinant antibody

ATG4C Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230359A4

Cat no : 84146-1-RR

Synonyms

AUTL3, AUTL1, ATG, APG4C, APG4 C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG4C Recombinant antibody (84146-1-RR)

    WB analysis using 84146-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84146-1-RR (ATG4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84146-1-RR

    Affinity and Kinetic Characterization of 84146-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84146-1-RR against Human ATG4C were performed. The affinity constant is 1.26 nM.

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84146-1-RR is ATG4C Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG4C fusion protein Ag14207
Full Name ATG4 autophagy related 4 homolog C (S. cerevisiae)
Calculated Molecular Weight 458 aa, 52 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC033024
Gene Symbol ATG4C
Gene ID (NCBI) 84938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Autophagy related 4C cysteine peptidase (ATG4C) is an autophagy regulator responsible for cleaving of pro-LC3 and delipidation of LC3 II.

Protocols

Product Specific Protocols
WB protocol for ATG4C antibody 84146-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols