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RAB9A Recombinant antibody

RAB9A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241372F8

Cat no : 84129-5-RR

Synonyms

RAB9, Ras-related protein Rab-9A, Ras related protein Rab 9A, 241372F8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB9A Recombinant antibody (84129-5-RR)

    WB analysis using 84129-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84129-5-RR (RAB9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using RAB9A Recombinant antibody (84129-5-RR)

    FC experiment of U2OS using 84129-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RAB9A Recombinant antibody (84129-5-RR, Clone:241372F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84129-5-RR

    Affinity and Kinetic Characterization of 84129-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84129-5-RR against Human RAB9A were performed. The affinity constant is 11.9 nM.

Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84129-5-RR is RAB9A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB9A fusion protein Ag1969
Full Name RAB9A, member RAS oncogene family
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC017265
Gene Symbol RAB9A
Gene ID (NCBI) 9367
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.

Protocols

Product Specific Protocols
WB protocol for RAB9A antibody 84129-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols