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  • Western Blot (WB) analysis of mouse eye tissue using Synaptotagmin-9 Recombinant antibody (84123-3-RR)

    WB analysis of mouse eye using 84123-3-RR

    mouse eye tissue was subjected to SDS PAGE followed by western blot with 84123-3-RR (Synaptotagmin-9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse retina tissue using Synaptotagmin-9 Recombinant antibody (84123-3-RR)

    IF Staining of mouse retina using 84123-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse retina tissue using Synaptotagmin-9 antibody (84123-3-RR, Clone: 241407A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84123-3-RR

    Affinity and Kinetic Characterization of 84123-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84123-3-RR against Human Synaptotagmin-9 were performed. The affinity constant is 22.5 nM.

Synaptotagmin-9 Recombinant antibody

Synaptotagmin-9 {ptg:Brand} Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84123-3-RR
Clone No.241407A11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

SytIX, SYT9, Synaptotagmin IX, 241407A11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse eye tissue
Positive IF-P detected inmouse retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84123-3-RR targets Synaptotagmin-9 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Synaptotagmin-9 fusion protein Ag3520 Predict reactive species
Full Name synaptotagmin IX
Calculated Molecular Weight 491 aa, 52 kDa
Observed Molecular Weight52-56 kDa
GenBank Accession NumberBC029605
Gene Symbol Synaptotagmin-9
Gene ID (NCBI) 143425
RRIDAB_3671684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86SS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-9 antibody 84123-3-RRDownload protocol
IF protocol for Synaptotagmin-9 antibody 84123-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols