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  • Western Blot (WB) analysis of various lysates using gp130/IL6ST Recombinant antibody (84113-5-RR)

    WB analysis using 84113-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84113-5-RR (gp130/IL6ST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84113-5-RR

    Affinity and Kinetic Characterization of 84113-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84113-5-RR against Rat gp130/IL6ST were performed. The affinity constant is 1.04 nM.

gp130/IL6ST Recombinant antibody

gp130/IL6ST {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 84113-5-RR
Clone No.241340A11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

IL6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130, CD130

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inrat spleen tissue, rat heart tissue, rat liver tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84113-5-RR targets gp130/IL6ST in WB, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein Predict reactive species
Full Name interleukin 6 signal transducer
Calculated Molecular Weight102 kDa
Observed Molecular Weight90-150 kDa
GenBank Accession NumberNM_001008725.3
Gene Symbol Il6st
Gene ID (NCBI) 25205
RRIDAB_3671678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40190
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).

Protocols

Product Specific Protocols
WB protocol for gp130/IL6ST antibody 84113-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols