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Rat gp130/IL6ST Recombinant antibody, PBS Only (Detector)

gp130/IL6ST Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241340A11

Cat no : 84113-5-PBS

Synonyms

IL-6RB, IL6RB, IL-6R subunit beta, IL-6 receptor subunit beta, CD130

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using gp130/IL6ST Recombinant antibody (84113-5-RR)

    WB analysis using 84113-5-RR (same clone as 84113-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84113-5-RR (gp130/IL6ST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84113-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01049-4

    Sandwich ELISA standard curve of MP01049-4

    Sandwich ELISA standard curve of MP01049-4, Rat gp130/IL6ST Recombinant Matched Antibody Pair - PBS only. 84113-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0857. 84113-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL

  • Sample test of MP01049-4

    Sample test of MP01049-4

    The mean gp130/IL6ST concentration was determined to be 24.02 ng/mL in C6 cell extract based on a 2.0 mg/mL extract load, 27.20 ng/mL in PC-12 cell extract based on a 1.4 mg/mL extract load and 16.64 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84113-5-RR

    Affinity and Kinetic Characterization of 84113-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84113-5-RR against Rat gp130/IL6ST were performed. The affinity constant is 1.04 nM.

Product Information

84113-5-PBS targets gp130/IL6ST as part of a matched antibody pair:

MP01049-4: 84113-2-PBS capture and 84113-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 6 signal transducer
Calculated Molecular Weight102 kDa
Observed Molecular Weight90-150 kDa
GenBank Accession NumberNM_001008725.3
Gene Symbol Il6st
Gene ID (NCBI) 25205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).