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Conjugate
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Conjugate
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Conjugate
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CCDC134 Recombinant antibody

CCDC134 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241212F11

Cat no : 84106-3-RR

Synonyms

241212F11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC134 Recombinant antibody (84106-3-RR)

    WB analysis using 84106-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using CCDC134 Recombinant antibody (84106-3-RR)

    FC experiment of U2OS using 84106-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84106-3-RR

    Affinity and Kinetic Characterization of 84106-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84106-3-RR against Human CCDC134 were performed. The affinity constant is 1.69 nM.

Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells, U-251 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84106-3-RR is CCDC134 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCDC134 fusion protein Ag21990
Full Name coiled-coil domain containing 134
Calculated Molecular Weight 229 aa, 27 kDa
Observed Molecular Weight25-27 kDa
GenBank Accession NumberBC017693
Gene Symbol CCDC134
Gene ID (NCBI) 79879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCDC134 antibody 84106-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols