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RBM10 Recombinant antibody

RBM10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241151G11

Cat no : 84104-4-RR

Synonyms

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM10 Recombinant antibody (84104-4-RR)

    WB analysis using 84104-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-4-RR (RBM10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM10 Recombinant antibody (84104-4-RR)

    IF Staining of A431 using 84104-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-4-RR, Clone: 241151G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, MDA-MB-231 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84104-4-RR is RBM10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM10 fusion protein Ag5788
Full Name RNA binding motif protein 10
Calculated Molecular Weight 104 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberBC024153
Gene Symbol RBM10
Gene ID (NCBI) 8241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

Protocols

Product Specific Protocols
WB protocol for RBM10 antibody 84104-4-RRDownload protocol
IF protocol for RBM10 antibody 84104-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols