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RBM10 Recombinant antibody

RBM10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241151D7

Cat no : 84104-2-RR

Synonyms

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM10 Recombinant antibody (84104-2-RR)

    WB analysis using 84104-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-2-RR (RBM10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    WB analysis of rat brain using 84104-2-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84104-2-RR (RBM10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    IHC staining of mouse brain using 84104-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    IHC staining of mouse brain using 84104-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-2-RR)

    IHC staining of mouse brain using 84104-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM10 Recombinant antibody (84104-2-RR)

    IF Staining of A431 using 84104-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-2-RR, Clone: 241151D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using RBM10 Recombinant antibody (84104-2-RR)

    FC experiment of A431 using 84104-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RBM10 Recombinant antibody (84104-2-RR, Clone:241151D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84104-2-RR is RBM10 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM10 fusion protein Ag5788
Full Name RNA binding motif protein 10
Calculated Molecular Weight 104 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberBC024153
Gene Symbol RBM10
Gene ID (NCBI) 8241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

Protocols

Product Specific Protocols
WB protocol for RBM10 antibody 84104-2-RRDownload protocol
IHC protocol for RBM10 antibody 84104-2-RRDownload protocol
IF protocol for RBM10 antibody 84104-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols