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PCF11 Recombinant antibody

PCF11 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241213F9

Cat no : 84081-4-RR

Synonyms

Pre-mRNA cleavage complex II protein Pcf11, Pre-mRNA cleavage complex 2 protein Pcf11, KIAA0824, 241213F9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCF11 Recombinant antibody (84081-4-RR)

    WB analysis using 84081-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84081-4-RR (PCF11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-2 OS using PCF11 Recombinant antibody (84081-4-RR)

    FC experiment of U-2 OS using 84081-4-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug PCF11 Recombinant antibody (84081-4-RR, Clone:241213F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, Neuro-2a cells, RAW 264.7 cells,
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84081-4-RR is PCF11 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCF11 fusion protein Ag20263
Full Name PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
Calculated Molecular Weight 1555 aa, 173 kDa
Observed Molecular Weight180~200 kDa
GenBank Accession NumberBC146778
Gene Symbol PCF11
Gene ID (NCBI) 51585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

Protocols

Product Specific Protocols
WB protocol for PCF11 antibody 84081-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols