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CD38 Recombinant antibody

CD38 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241246C2

Cat no : 84070-5-RR

Synonyms

ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD38 Recombinant antibody (84070-5-RR)

    WB analysis using 84070-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84070-5-RR (CD38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells, Jurkat cells, A549 cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84070-5-RR is CD38 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD38 molecule
Calculated Molecular Weight 300 aa, 34 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC007964
Gene Symbol CD38
Gene ID (NCBI) 952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Protocols

Product Specific Protocols
WB protocol for CD38 antibody 84070-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols