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IDO1 Recombinant antibody, PBS Only (Detector)

IDO1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241110F5

Cat no : 84063-4-PBS

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00982-3

    Sandwich ELISA standard curve of MP00982-3

    Sandwich ELISA standard curve of MP00982-3, Human IDO1 Recombinant Matched Antibody Pair - PBS only. 84063-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3953. 84063-4-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP00982-3

    Sample test of MP00982-3

    A549 cells were cultured unstimulated or stimulated with 300 μg/mL IFN-gamma for 5 days. The mean IDO1 concentration was determined to be 0.30 ng/mL in unstimulated A549 supernatant, 1.19 ng/mL in IFN-gamma stimulated A549 supernatant.

  • Sample test of MP00982-3

    Sample test of MP00982-3

    Serum of fifteen individual healthy human donors was measured. The human IDO1 concentration of detected samples was determined to be 8.21 ng/mL with a range of ND - 41.58 ng/mL. (ND = Non-detectable)

  • Cytometric bead array standard curve of MP00982-1

    Cytometric bead array standard curve of MP00982-1

    Cytometric bead array standard curve of MP00982-1, IDO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84063-2-PBS. Detection antibody: 84063-4-PBS. Standard: Ag3953. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00982-3

    Cytometric bead array standard curve of MP00982-3

    Cytometric bead array standard curve of MP00982-3, IDO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84063-1-PBS. Detection antibody: 84063-4-PBS. Standard: Ag3953. Range: 1.25-40 ng/mL.

Product Information

84063-4-PBS targets IDO1 as part of a matched antibody pair:

MP00982-1: 84063-2-PBS capture and 84063-4-PBS detection (validated in Cytometric bead array)

MP00982-3: 84063-1-PBS capture and 84063-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IDO1 fusion protein Ag3953
Full Name indoleamine 2,3-dioxygenase 1
Calculated Molecular Weight 403 aa, 45 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.