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IDO1 Recombinant antibody

IDO1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241110C8

Cat no : 84063-1-RR

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IDO1 Recombinant antibody (84063-1-RR)

    WB analysis of HeLa using 84063-1-RR

    IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 84063-1-RR (IDO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using IDO1 Recombinant antibody (84063-1-RR)

    FC experiment of HeLa using 84063-1-RR

    1x10^6 IFN-gamma treated HeLa cells were intracellularly stained with 0.25 ug Ido1 Recombinant Antibody (84063-1-RR, Clone:241110C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84063-1-RR

    Affinity and Kinetic Characterization of 84063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84063-1-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inIFN gamma treated HeLa cells
Positive FC (Intra) detected inIFN-gamma treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84063-1-RR is IDO1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IDO1 fusion protein Ag3953
Full Name indoleamine 2,3-dioxygenase 1
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Protocols

Product Specific Protocols
WB protocol for IDO1 antibody 84063-1-RRDownload protocol
FC protocol for IDO1 antibody 84063-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols