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KLK3/PSA Recombinant antibody

KLK3/PSA Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241223E9

Cat no : 84059-7-RR

Synonyms

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using KLK3/PSA Recombinant antibody (84059-7-RR)

    WB analysis of LNCaP using 84059-7-RR

    LNCaP cells were subjected to SDS PAGE followed by western blot with 84059-7-RR (KLK3/PSA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84059-7-RR

    Affinity and Kinetic Characterization of 84059-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84059-7-RR against Human KLK3/PSA were performed. The affinity constant is 0.307 nM.

Tested Applications

Positive WB detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84059-7-RR is KLK3/PSA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name kallikrein-related peptidase 3
Calculated Molecular Weight29 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberNM_001648.2
Gene Symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for KLK3/PSA antibody 84059-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols