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RREB1-Specific Recombinant antibody

RREB1-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240800D6

Cat no : 84048-2-RR

Synonyms

RREB1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321, HNT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RREB1-Specific Recombinant antibody (84048-2-RR)

    WB analysis using 84048-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84048-2-RR (RREB1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-2 OS using RREB1-Specific Recombinant antibody (84048-2-RR)

    FC experiment of U-2 OS using 84048-2-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug RREB1-Specific Recombinant antibody (84048-2-RR, Clone:240800D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84048-2-RR targets RREB1-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name ras responsive element binding protein 1
Calculated Molecular Weight 181 kDa
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_001003698
Gene Symbol RREB1
Gene ID (NCBI) 6239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

Protocols

Product Specific Protocols
WB protocol for RREB1-Specific antibody 84048-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols