Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84033-2-RR (ACSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 84033-2-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A549 using 84033-2-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A549 cells
Recommended dilution
Application
Dilution
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84033-2-RR targets ACSF2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84033-2-RR (ACSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A549 using 84033-2-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A549 using 84033-2-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
AFFINITY Figures
Affinity and Kinetic Characterization of 84033-2-RR
Biolayer interferometry (BLl) kinetic assays of 84033-2-RR against Human ACSF2 were performed. The affinity constant is 0.595 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
ACSF2 Recombinant antibody
Catalog Number
84033-2-RR
Citations
-
Dilutions
IHC : 1:250-1:1000 IF/ICC : 1:200-1:800
Applications
IHC, IF/ICC, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
