Various lysates were subjected to SDS PAGE followed by western blot with 84031-5-RR (HINT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84031-5-RR (HINT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 84031-5-RR
WB result of HINT2 antibody (84031-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT2 transfected HeLa cells.
WB result of HINT2 antibody (84031-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT2 transfected HeLa cells.
IF Staining of HeLa using 84031-5-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (84031-5-RR, Clone: 241150D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (84031-5-RR, Clone: 241150D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), (red).
Affinity and Kinetic Characterization of 84031-5-RR
Biolayer interferometry (BLl) kinetic assays of 84031-5-RR against Human HINT2 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 84031-5-RR (HINT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 84031-5-RR
WB result of HINT2 antibody (84031-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT2 transfected HeLa cells.
IF/ICC Figures
IF Staining of HeLa using 84031-5-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (84031-5-RR, Clone: 241150D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), (red).
AFFINITY Figures
Affinity and Kinetic Characterization of 84031-5-RR
Biolayer interferometry (BLl) kinetic assays of 84031-5-RR against Human HINT2 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
HINT2 Recombinant antibody
Catalog Number
84031-5-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 IF/ICC : 1:250-1:1000
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
