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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOXF1 Recombinant antibody (84020-3-RR)

    IF Staining of HeLa using 84020-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOXF1 Recombinant antibody (84020-3-RR)

    IF Staining of HeLa using 84020-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXF1 antibody (84020-3-RR, Clone: 241076C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using FOXF1 Recombinant antibody (84020-3-RR)

    FC experiment of HeLa using 84020-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXF1 Recombinant antibody (84020-3-RR, Clone:241076C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84020-3-RR

    Affinity and Kinetic Characterization of 84020-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84020-3-RR against Human FOXF1 were performed. The affinity constant is below 1 pM.

FOXF1 Recombinant antibody

FOXF1 {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84020-3-RR
Clone No.241076C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

241076C8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84020-3-RR targets FOXF1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXF1 fusion protein Ag34266 Predict reactive species
Full Name forkhead box F1
Calculated Molecular Weight40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberNP_001442
Gene Symbol FOXF1
Gene ID (NCBI) 2294
RRIDAB_3671589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12946
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXF1 (Forkhead box protein F1), also known as FKHL5, It is expected to be located in the nucleus, which is expressed predominantly in lung and placenta. FOXF1, a member of the Forkhead box family of transcription factors, as one of the most highly and consistently upregulated genes in FP-RMS. While it is known that FOXF1 is required for mesodermal differentiation toward endothelial and smooth muscle cell lineages, FOXF1 is not expressed in differentiated skeletal muscle cells, and its role in fusion-positive FP-RMS remains unknown (PMID: 33627785). The molecular weight of FOXF1 is 40 kDa.

Protocols

Product Specific Protocols
IF protocol for FOXF1 antibody 84020-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols