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  • Western Blot (WB) analysis of various lysates using EXOC1 Recombinant antibody (84007-5-RR)

    WB analysis using 84007-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84007-5-RR (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EXOC1 Recombinant antibody (84007-5-RR)

    IHC staining of human placenta using 84007-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84007-5-RR (EXOC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using EXOC1 Recombinant antibody (84007-5-RR)

    IF Staining of human placenta using 84007-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (84007-5-RR, Clone: 241058D6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using EXOC1 Recombinant antibody (84007-5-RR)

    IF Staining of human placenta using 84007-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (84007-5-RR, Clone: 241058D6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using EXOC1 Recombinant antibody (84007-5-RR)

    IF Staining of human placenta using 84007-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (84007-5-RR, Clone: 241058D6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EXOC1 Recombinant antibody

EXOC1 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84007-5-RR
Clone No.241058D6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

241058D6, BM 102, Exocyst complex component 1, Exocyst complex component Sec3, SEC3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, C1C12 cells, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84007-5-RR targets EXOC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EXOC1 fusion protein Ag2303 Predict reactive species
Full Name exocyst complex component 1
Calculated Molecular Weight 894 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC020650
Gene Symbol EXOC1
Gene ID (NCBI) 55763
RRIDAB_3671576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NV70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Protocols

Product Specific Protocols
WB protocol for EXOC1 antibody 84007-5-RRDownload protocol
IHC protocol for EXOC1 antibody 84007-5-RRDownload protocol
IF protocol for EXOC1 antibody 84007-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols