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E-cadherin Recombinant antibody, PBS Only (Capture)

E-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241098G6

Cat no : 83991-4-PBS

Synonyms

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using E-cadherin Recombinant antibody (83991-4-RR)

    WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using E-cadherin Recombinant antibody (83991-4-RR)

    WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using E-cadherin Recombinant antibody (83991-4-RR)

    IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using E-cadherin Recombinant antibody (83991-4-RR)

    IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using E-cadherin Recombinant antibody (83991-4-RR)

    IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using E-cadherin Recombinant antibody (83991-4-RR)

    IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using E-cadherin Recombinant antibody (83991-4-RR)

    IF Staining of A431 using 83991-4-RR (same clone as 83991-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using E-cadherin Recombinant antibody (83991-4-RR)

    IF Staining of MCF-7 using 83991-4-RR (same clone as 83991-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00187-6

    Cytometric bead array standard curve of MP00187-6

    Cytometric bead array standard curve of MP00187-6, E-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83991-4-PBS. Detection antibody: 83991-2-PBS. Standard: Eg1112. Range: 0.313-40 ng/mL.

Product Information

83991-4-PBS targets E-cadherin as part of a matched antibody pair:

MP00187-6: 83991-4-PBS capture and 83991-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight97 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberNM_004360.5
Gene Symbol E-cadherin
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.