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NFE2L1 Recombinant antibody

NFE2L1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241089D1

Cat no : 83987-2-RR

Synonyms

NF-E2-related factor 1, Locus control region-factor 1, LCR-F1, HBZ17, Endoplasmic reticulum membrane sensor NFE2L1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFE2L1 Recombinant antibody (83987-2-RR)

    IF Staining of HepG2 using 83987-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFE2L1 antibody (83987-2-RR, Clone: 241089D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83987-2-RR

    Affinity and Kinetic Characterization of 83987-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83987-2-RR against Human NFE2L1 were performed. The affinity constant is 0.988 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83987-2-RR is NFE2L1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFE2L1 fusion protein Ag0525
Full Name nuclear factor (erythroid-derived 2)-like 1
Calculated Molecular Weight 772 aa, 85 kDa
GenBank Accession NumberBC010623
Gene Symbol NFE2L1
Gene ID (NCBI) 4779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for NFE2L1 antibody 83987-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols