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AGXT2L1 Recombinant antibody

AGXT2L1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241119H5

Cat no : 83984-5-RR

Synonyms

ETNPPL, Ethanolamine-phosphate phospho-lyase, EC:4.2.3.2, Alanine--glyoxylate aminotransferase 2-like 1, 241119H5

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using AGXT2L1 Recombinant antibody (83984-5-RR)

    IHC staining of mouse liver using 83984-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83984-5-RR (AGXT2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AGXT2L1 Recombinant antibody (83984-5-RR)

    IHC staining of mouse liver using 83984-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83984-5-RR (AGXT2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using AGXT2L1 Recombinant antibody (83984-5-RR)

    IF Staining of U2OS using 83984-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AGXT2L1 antibody (83984-5-RR, Clone: 241119H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83984-5-RR

    Affinity and Kinetic Characterization of 83984-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83984-5-RR against Human AGXT2L1 were performed. The affinity constant is 1.77 nM.

Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83984-5-RR is AGXT2L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AGXT2L1 fusion protein Ag10584
Full Name alanine-glyoxylate aminotransferase 2-like 1
Calculated Molecular Weight 499 aa, 56 kDa
GenBank Accession NumberBC022526
Gene Symbol AGXT2L1
Gene ID (NCBI) 64850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768,

Protocols

Product Specific Protocols
IHC protocol for AGXT2L1 antibody 83984-5-RRDownload protocol
IF protocol for AGXT2L1 antibody 83984-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols