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  • Western Blot (WB) analysis of various lysates using Perforin Recombinant antibody (83977-1-RR)

    WB analysis using 83977-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83977-1-RR (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83977-1-RR

    Affinity and Kinetic Characterization of 83977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83977-1-RR against Human Perforin were performed. The affinity constant is 1.05 nM.

Perforin Recombinant antibody

Perforin {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 83977-1-RR
Clone No.241009C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

241009C10, Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inCTLL-2 cells, NK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83977-1-RR targets Perforin in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Perforin fusion protein Ag6060 Predict reactive species
Full Name perforin 1 (pore forming protein)
Calculated Molecular Weight 61 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC063043
Gene Symbol Perforin
Gene ID (NCBI) 5551
RRIDAB_3671552
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Perforin (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)

Protocols

Product Specific Protocols
WB protocol for Perforin antibody 83977-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols