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CDK7 Recombinant antibody

CDK7 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240962F9

Cat no : 83976-3-RR

Synonyms

CDK-activating kinase 1, CDK activating kinase, CAK1, CAK, 39 kDa protein kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK7 Recombinant antibody (83976-3-RR)

    WB analysis using 83976-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83976-3-RR (CDK7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83976-3-RR

    Affinity and Kinetic Characterization of 83976-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83976-3-RR against Human CDK7 were performed. The affinity constant is 28.7 nM.

Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83976-3-RR is CDK7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK7 fusion protein Ag25737
Full Name cyclin-dependent kinase 7
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC005298
Gene Symbol CDK7
Gene ID (NCBI) 1022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDK7 antibody 83976-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols