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  • Featured Product
  • KD/KO Validated

PEA15 Recombinant antibody, PBS Only (Capture)

PEA15 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241044G8

Cat no : 83958-2-PBS

Synonyms

MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEA15 Recombinant antibody (83958-2-RR)

    WB analysis using 83958-2-RR (same clone as 83958-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83958-2-RR (PEA15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PEA15 Recombinant antibody (83958-2-RR)

    WB analysis of COLO 320 using 83958-2-RR (same clone as 83958-2-PBS)

    WB result of PEA15 antibody (83958-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEA15 transfected COLO 320 cells. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00916-1

    Cytometric bead array standard curve of MP00916-1

    Cytometric bead array standard curve of MP00916-1, PEA15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83958-2-PBS. Detection antibody: 83958-1-PBS. Standard: Ag13782. Range: 3.125-200 ng/mL.

Product Information

83958-2-PBS targets PEA15 as part of a matched antibody pair:

MP00916-1: 83958-2-PBS capture and 83958-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEA15 fusion protein Ag13782
Full Name phosphoprotein enriched in astrocytes 15
Calculated Molecular Weight 130 aa, 15 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC002426
Gene Symbol PEA15
Gene ID (NCBI) 8682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.