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CXXC5 Recombinant antibody

CXXC5 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241034E6

Cat no : 83940-6-RR

Synonyms

Putative MAPK-activating protein PM08, HSPC195, CXXC-type zinc finger protein 5, CXXC finger 5, CF5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CXXC5 Recombinant antibody (83940-6-RR)

    IF Staining of HepG2 using 83940-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CXXC5 antibody (83940-6-RR, Clone: 241034E6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83940-6-RR

    Affinity and Kinetic Characterization of 83940-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83940-6-RR against Human CXXC5 were performed. The affinity constant is 6.36 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83940-6-RR is CXXC5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXXC5 fusion protein Ag9733
Full Name CXXC finger 5
Calculated Molecular Weight322aa,33 kDa; 227aa,24 kDa
GenBank Accession NumberBC017439
Gene Symbol CXXC5
Gene ID (NCBI) 51523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

Protocols

Product Specific Protocols
IF protocol for CXXC5 antibody 83940-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols