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CXCL1 Recombinant antibody

CXCL1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240990E4

Cat no : 83937-3-RR

Synonyms

GRO-alpha(5-73), GRO-alpha(4-73), CXCL 1, C-X-C motif chemokine 1, C X C motif chemokine 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using CXCL1 Recombinant antibody (83937-3-RR)

    WB analysis of HUVEC using 83937-3-RR

    Untreated, LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83937-3-RR (CXCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83937-3-RR

    Affinity and Kinetic Characterization of 83937-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83937-3-RR against Human CXCL1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83937-3-RR is CXCL1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXCL1 fusion protein Ag2917
Full Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Calculated Molecular Weight 8 kDa to 14 kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberBC011976
Gene Symbol CXCL1
Gene ID (NCBI) 2919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Protocols

Product Specific Protocols
WB protocol for CXCL1 antibody 83937-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols