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NUCB2/nesfatin-1 Recombinant antibody

NUCB2/nesfatin-1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241037D8

Cat no : 83917-2-RR

Synonyms

NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    WB analysis using 83917-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    WB analysis using 83917-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    FC experiment of A431 using 83917-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    FC experiment of HepG2 using 83917-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83917-2-RR

    Affinity and Kinetic Characterization of 83917-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83917-2-RR against Human NUCB2/nesfatin-1 were performed. The affinity constant is 8.99 nM.

Tested Applications

Positive WB detected inMCF-7 cells, mouse testis tissue, C6 cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83917-2-RR is NUCB2/nesfatin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUCB2/nesfatin-1 fusion protein Ag25152
Full Name nucleobindin 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_005013
Gene Symbol NUCB2
Gene ID (NCBI) 4925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Protocols

Product Specific Protocols
WB protocol for NUCB2/nesfatin-1 antibody 83917-2-RRDownload protocol
FC protocol for NUCB2/nesfatin-1 antibody 83917-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols