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NDRG2 Recombinant antibody

NDRG2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241039E8

Cat no : 83902-7-RR

Synonyms

SYLD, Protein Syld709613, Protein NDRG2, N-myc downstream-regulated gene 2 protein, NDRG family member 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDRG2 Recombinant antibody (83902-7-RR)

    WB analysis using 83902-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83902-7-RR (NDRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDRG2 Recombinant antibody (83902-7-RR)

    IHC staining of mouse brain using 83902-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDRG2 Recombinant antibody (83902-7-RR)

    IHC staining of mouse brain using 83902-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using NDRG2 Recombinant antibody (83902-7-RR)

    FC experiment of HepG2 using 83902-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDRG2 Recombinant antibody (83902-7-RR, Clone:241039E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83902-7-RR

    Affinity and Kinetic Characterization of 83902-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83902-7-RR against Human NDRG2 were performed. The affinity constant is 0.114 nM.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue, human heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83902-7-RR is NDRG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NDRG2 fusion protein Ag2629
Full Name NDRG family member 2
Calculated Molecular Weight 371 aa, 39 kDa
Observed Molecular Weight41-45 kDa
GenBank Accession NumberBC010458
Gene Symbol NDRG2
Gene ID (NCBI) 57447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-myc downstream-regulated gene 2 (NDRG2) is a newly identified member of the NDRG family that is highly localized and plays an important role in the regulation of astrocyte function. In astrocytes, NDRG2 affects the regulation of apoptosis, astrogliosis, blood-brain barrier integrity, and glutamate clearance. Several preclinical studies have revealed that NDRG2 is implicated in the pathogenesis of many neurological diseases, such as stroke, neurodegeneration and psychiatric disorders. Recent studies demonstrated that NDRG2 functions as a tumor suppressor and may be a prognostic predictor for many different malignant tumors.

Protocols

Product Specific Protocols
WB protocol for NDRG2 antibody 83902-7-RRDownload protocol
IHC protocol for NDRG2 antibody 83902-7-RRDownload protocol
FC protocol for NDRG2 antibody 83902-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols