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VPRBP Recombinant antibody

VPRBP Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241028B4

Cat no : 83901-5-RR

Synonyms

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPRBP Recombinant antibody (83901-5-RR)

    IF Staining of HeLa using 83901-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U-2 OS using VPRBP Recombinant antibody (83901-5-RR)

    FC experiment of U-2 OS using 83901-5-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83901-5-RR

    Affinity and Kinetic Characterization of 83901-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83901-5-RR against Human VPRBP were performed. The affinity constant is 44 pM.

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83901-5-RR is VPRBP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VPRBP fusion protein Ag2184
Full Name Vpr (HIV-1) binding protein
Calculated Molecular Weight 1506 aa, 169 kDa
Observed Molecular Weight169 kDa
GenBank Accession NumberBC022792
Gene Symbol VPRBP
Gene ID (NCBI) 9730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Protocols

Product Specific Protocols
IF protocol for VPRBP antibody 83901-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols