• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse NCAM1/CD56 Recombinant antibody, PBS Only (Capture)

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240936B9

Cat no : 83883-2-PBS

Synonyms

Ncam1, CD56, 240936B9, NCAM 1, Ncam

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of C6 cells using NCAM1/CD56 Recombinant antibody (83883-2-RR)

    WB analysis of C6 using 83883-2-RR (same clone as 83883-2-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 83883-2-RR (Ncam1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83883-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00841-1

    Cytometric bead array standard curve of MP00841-1

    Cytometric bead array standard curve of MP00841-1, MOUSE NCAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83883-2-PBS. Detection antibody: 83883-4-PBS. Standard: Eg0652. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 83883-2-RR

    Affinity and Kinetic Characterization of 83883-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83883-2-RR against Mouse Ncam1 were performed. The affinity constant is 0.833 nM.

Product Information

83883-2-PBS targets NCAM1/CD56 as part of a matched antibody pair:

MP00841-1: 83883-2-PBS capture and 83883-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name neural cell adhesion molecule 1
Calculated Molecular Weight119 kDa
Observed Molecular Weight140 kDa
Gene Symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).