• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IRAK3 Recombinant antibody

IRAK3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240975C5

Cat no : 83876-2-RR

Synonyms

IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, ASRT5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of THP-1 cells using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of THP-1 using 83876-2-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of mouse lung using 83876-2-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis using 83876-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83876-2-RR is IRAK3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IRAK3 fusion protein Ag4959
Full Name interleukin-1 receptor-associated kinase 3
Calculated Molecular Weight 68 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC057800
Gene Symbol IRAK3
Gene ID (NCBI) 11213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).

Protocols

Product Specific Protocols
WB protocol for IRAK3 antibody 83876-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols