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UCP1 Recombinant antibody

UCP1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240782F3

Cat no : 83870-1-RR

Synonyms

UCP 1, UCP, Thermogenin, SLC25A7, 240782F3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    WB analysis of mouse brown adipose using 83870-1-RR

    mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83870-1-RR

    Affinity and Kinetic Characterization of 83870-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

Tested Applications

Positive WB detected inmouse brown adipose tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83870-1-RR is UCP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCP1 fusion protein Ag20388
Full Name uncoupling protein 1 (mitochondrial, proton carrier)
Calculated Molecular Weight 307 aa, 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC098168
Gene Symbol UCP1
Gene ID (NCBI) 7350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

Protocols

Product Specific Protocols
WB protocol for UCP1 antibody 83870-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols