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PADI2 Recombinant antibody

PADI2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240763C5

Cat no : 83868-1-RR

Synonyms

PAD-H19, PAD2, PAD H19, KIAA0994, EC:3.5.3.15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PADI2 Recombinant antibody (83868-1-RR)

    WB analysis using 83868-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83868-1-RR (PADI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83868-1-RR

    Affinity and Kinetic Characterization of 83868-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83868-1-RR against Human PADI2 were performed. The affinity constant is 1.01 nM.

Tested Applications

Positive WB detected inMCF-7 cells, HL-60 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83868-1-RR is PADI2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PADI2 fusion protein Ag17612
Full Name peptidyl arginine deiminase, type II
Calculated Molecular Weight 665 aa, 75 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC009701
Gene Symbol PADI2
Gene ID (NCBI) 11240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PADI2, also named KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670).

Protocols

Product Specific Protocols
WB protocol for PADI2 antibody 83868-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols