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  • Immunohistochemistry (IHC) staining of mouse skin tissue using Loricrin Recombinant antibody (83865-1-RR)

    IHC staining of mouse skin using 83865-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Loricrin Recombinant antibody (83865-1-RR)

    IHC staining of mouse skin using 83865-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Loricrin Recombinant antibody (83865-1-RR)

    IF Staining of A431 using 83865-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LOR antibody (83865-1-RR, Clone: 240605C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using Loricrin Recombinant antibody (83865-1-RR)

    FC experiment of A431 using 83865-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Loricrin Recombinant antibody (83865-1-RR, Clone:240605C8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Loricrin Recombinant antibody

Loricrin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83865-1-RR
Clone No.240605C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

LOR, 240605C8, LRN

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83865-1-RR targets Loricrin in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name loricrin
Calculated Molecular Weight 26 kDa
GenBank Accession NumberNM_000427
Gene Symbol Loricrin
Gene ID (NCBI) 4014
RRIDAB_3671451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.

Protocols

Product Specific Protocols
IHC protocol for Loricrin antibody 83865-1-RRDownload protocol
IF protocol for Loricrin antibody 83865-1-RRDownload protocol
FC protocol for Loricrin antibody 83865-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols