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ATP2B1 Recombinant antibody

ATP2B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240848D10

Cat no : 83827-3-RR

Synonyms

PMCA1kb, PMCA1, 240848D10

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATP2B1 Recombinant antibody (83827-3-RR)

    WB analysis of mouse brain using 83827-3-RR

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ATP2B1 Recombinant antibody (83827-3-RR)

    IHC staining of mouse small intestine using 83827-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83827-3-RR

    Affinity and Kinetic Characterization of 83827-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

Tested Applications

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83827-3-RR is ATP2B1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP2B1 fusion protein Ag32423
Full Name ATPase, Ca++ transporting, plasma membrane 1
Calculated Molecular Weight135 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001001323.1
Gene Symbol ATP2B1
Gene ID (NCBI) 490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).

Protocols

Product Specific Protocols
WB protocol for ATP2B1 antibody 83827-3-RRDownload protocol
IHC protocol for ATP2B1 antibody 83827-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols