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CPNE3 Recombinant antibody

CPNE3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

240871E5

Cat no : 83820-5-RR

Synonyms

CPN3, copineIII, Copine-3, Copine III, Copine 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPNE3 Recombinant antibody (83820-5-RR)

    WB analysis using 83820-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83820-5-RR (CPNE3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CPNE3 Recombinant antibody (83820-5-RR)

    IP experiment of HepG2 using 83820-5-RR

    IP result of anti-CPNE3 (IP:83820-5-RR, 4ug; Detection:83820-5-RR 1:2000) with HepG2 cells lysate 1600 ug.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CPNE3 Recombinant antibody (83820-5-RR)

    IF Staining of A431 using 83820-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPNE3 antibody (83820-5-RR, Clone: 240871E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CPNE3 Recombinant antibody (83820-5-RR)

    FC experiment of HepG2 using 83820-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPNE3 Recombinant antibody (83820-5-RR, Clone:240871E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83820-5-RR

    Affinity and Kinetic Characterization of 83820-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83820-5-RR against Human CPNE3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inY79 cells, HepG2 cells, A431 cells, A549 cells, HeLa cells, HuH-7 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83820-5-RR is CPNE3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPNE3 fusion protein Ag1634
Full Name copine III
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC036242
Gene Symbol CPNE3
Gene ID (NCBI) 8895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. Copine III (CPNE3) has also been identified as a ligand of ERBB2 and plays an important role in various carcinogenesis. The high expression of CPNE3 is an adverse prognostic biomarker for acute myeloid leukemia (PMID: 28670859).

Protocols

Product Specific Protocols
WB protocol for CPNE3 antibody 83820-5-RRDownload protocol
IF protocol for CPNE3 antibody 83820-5-RRDownload protocol
IP protocol for CPNE3 antibody 83820-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols