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Phospho-Histone H2B (Ser14) Recombinant antibody

Phospho-Histone H2B (Ser14) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240570A8

Cat no : 83811-1-RR

Synonyms

H2B (Ser14), Histone H2B (Ser14), p H2B, p H2B (Ser14), p Histone H2B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Histone H2B (Ser14) Recombinant antibody (83811-1-RR)

    WB analysis using 83811-1-RR

    Non-treated NIH/3T3 cells and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83811-1-RR (Phospho-Histone H2B (Ser14)) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Tested Applications

Positive WB detected inPMA treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83811-1-RR targets Phospho-Histone H2B (Ser14) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 2, H2be
Calculated Molecular Weight 14 kDa
Observed Molecular Weight14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. In addition, MST1 is known to phosphorylate histone H2B in vitro and has recently been shown to directly phosphorylate histone H2B at Ser14 in vivo. (PMID: 17548476)

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H2B (Ser14) antibody 83811-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols