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CSNK2A1 Recombinant antibody

CSNK2A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240862A7

Cat no : 83805-4-RR

Synonyms

CKII, CK2α, CK2A1, CK2a, CK II alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK2A1 Recombinant antibody (83805-4-RR)

    WB analysis using 83805-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83805-4-RR (CSNK2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSNK2A1 Recombinant antibody (83805-4-RR)

    IF Staining of HeLa using 83805-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSNK2A1 Recombinant antibody (83805-4-RR)

    IF Staining of HeLa using 83805-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Jurkat cells using CSNK2A1 Recombinant antibody (83805-4-RR)

    FC experiment of Jurkat using 83805-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CSNK2A1 Recombinant antibody (83805-4-RR, Clone:240862A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83805-4-RR

    Affinity and Kinetic Characterization of 83805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83805-4-RR against Human CSNK2A1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293T cells, Raji cells, SH-SY5Y cells, U-251 cells, A431 cells, C6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83805-4-RR is CSNK2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CSNK2A1 fusion protein Ag1452
Full Name casein kinase 2, alpha 1 polypeptide
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC011668
Gene Symbol CSNK2A1
Gene ID (NCBI) 1457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Protocols

Product Specific Protocols
WB protocol for CSNK2A1 antibody 83805-4-RRDownload protocol
IF protocol for CSNK2A1 antibody 83805-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols