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5 Lipoxygenase Recombinant antibody

5 Lipoxygenase Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240740A10

Cat no : 83794-4-RR

Synonyms

ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 5 Lipoxygenase Recombinant antibody (83794-4-RR)

    WB analysis using 83794-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83794-4-RR (ALOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using 5 Lipoxygenase Recombinant antibody (83794-4-RR)

    IHC staining of human ovarian cancer using 83794-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83794-4-RR (5 Lipoxygenase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83794-4-RR

    Affinity and Kinetic Characterization of 83794-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83794-4-RR against Human 5 Lipoxygenase were performed. The affinity constant is 0.392 nM.

Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells, human placenta tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83794-4-RR is 5 Lipoxygenase Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen 5 Lipoxygenase fusion protein Ag19503
Full Name arachidonate 5-lipoxygenase
Calculated Molecular Weight 674 aa, 78 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC130332
Gene Symbol 5 Lipoxygenase
Gene ID (NCBI) 240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Protocols

Product Specific Protocols
WB protocol for 5 Lipoxygenase antibody 83794-4-RRDownload protocol
IHC protocol for 5 Lipoxygenase antibody 83794-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols