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GPD1L Recombinant antibody

GPD1L Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240846F9

Cat no : 83792-2-RR

Synonyms

GPD1-L, GPD1 L, Glycerol-3-phosphate dehydrogenase 1-like protein, EC:1.1.1.8, 240846F9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPD1L Recombinant antibody (83792-2-RR)

    WB analysis using 83792-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83792-2-RR (GPD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83792-2-RR

    Affinity and Kinetic Characterization of 83792-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83792-2-RR against Human GPD1L were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, MDA-MB-231 cells, NCI-H1299 cells, mouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83792-2-RR is GPD1L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPD1L fusion protein Ag10977
Full Name glycerol-3-phosphate dehydrogenase 1-like
Calculated Molecular Weight 351 aa, 38 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC028726
Gene Symbol GPD1L
Gene ID (NCBI) 23171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is encoded by the gene GPD1L, which is located on chromosome 3p22.3. GPD1L is responsible for catalyzing the conversion of sn-glycerol 3-phosphate to glycerone phosphate. GPD1L displayed a dysregulated state in a wide variety of cancers.

Protocols

Product Specific Protocols
WB protocol for GPD1L antibody 83792-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols