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TDG Recombinant antibody, PBS Only (Detector)

TDG Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240715E12

Cat no : 83780-3-PBS

Synonyms

Thymine-DNA glycosylase, thymine DNA glycosylase, hTDG, G/T mismatch-specific thymine DNA glycosylase, EC:3.2.2.29

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TDG Recombinant antibody (83780-3-RR)

    IF Staining of HepG2 using 83780-3-RR (same clone as 83780-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TDG antibody (83780-3-RR, Clone: 240715E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83780-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TDG Recombinant antibody (83780-3-RR)

    FC experiment of HeLa using 83780-3-RR (same clone as 83780-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TDG Recombinant Antibody (83780-3-RR, Clone:240715E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 83780-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00744-1

    Cytometric bead array standard curve of MP00744-1

    Cytometric bead array standard curve of MP00744-1, TDG Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83780-1-PBS. Detection antibody: 83780-3-PBS. Standard: Ag4190. Range: 1.25-80 ng/mL.

Product Information

83780-3-PBS targets TDG as part of a matched antibody pair:

MP00744-1: 83780-1-PBS capture and 83780-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDG fusion protein Ag4190
Full Name thymine-DNA glycosylase
Calculated Molecular Weight 410 aa, 46 kDa
GenBank Accession NumberBC037557
Gene Symbol TDG
Gene ID (NCBI) 6996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.