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VE-cadherin/CD144 Recombinant antibody

VE-cadherin/CD144 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240755D5

Cat no : 83766-6-RR

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Recombinant antibody (83766-6-RR)

    WB analysis using 83766-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83766-6-RR (VE-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VE-cadherin/CD144 Recombinant antibody (83766-6-RR)

    IF Staining of human placenta using 83766-6-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin/CD144 antibody (83766-6-RR, Clone: 240755D5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83766-6-RR is VE-cadherin/CD144 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated Molecular Weight88 kDa
Observed Molecular Weight90-100 kDa, 130-140 kDa
GenBank Accession NumberNM_001795.5
Gene Symbol VE-cadherin
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

Protocols

Product Specific Protocols
WB protocol for VE-cadherin/CD144 antibody 83766-6-RRDownload protocol
IF protocol for VE-cadherin/CD144 antibody 83766-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols