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  • Western Blot (WB) analysis of NR8383 cells using Cxcl2 Recombinant antibody (83758-1-RR)

    WB analysis of NR8383 using 83758-1-RR

    LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83758-1-RR (Cxcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Cxcl2 Recombinant antibody (83758-1-RR)

    IF Staining of THP-1 using 83758-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using Cxcl2 antibody (83758-1-RR, Clone: 240367C5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83758-1-RR

    Affinity and Kinetic Characterization of 83758-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83758-1-RR against Mouse Cxcl2 were performed. The affinity constant is 0.517 nM.

Cxcl2 Recombinant antibody

Cxcl2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83758-1-RR
Clone No.240367C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

240367C5, CINC-3, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inLPS and Brefeldin A treated NR8383 cells
Positive IF/ICC detected inPMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83758-1-RR targets Cxcl2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name chemokine (C-X-C motif) ligand 2
Calculated Molecular Weight11 kDa
Observed Molecular Weight10 kDa
GenBank Accession NumberNM_053647.1
Gene Symbol Cxcl2
Gene ID (NCBI) 114105
RRIDAB_3671353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP30348
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).

Protocols

Product Specific Protocols
WB protocol for Cxcl2 antibody 83758-1-RRDownload protocol
IF protocol for Cxcl2 antibody 83758-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols